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mouse htr2a monoclonal antibody  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology mouse htr2a monoclonal antibody
    (A) Representative image of <t>HTR2A</t> protein immunoblot with increasing amounts of total protein (10 μg, 12 μg, 14 μg, 16 μg, 18 μg, 20 μg). (B) Linear regression of pooled total protein extracted from bovine lateral saphenous veins with HTR2A protein expression. (B) Representative image of HTR2A protein immunoblot with increasing amounts of total protein. (C) Representative image of HTR2A protein immunoblot for quantitative protein expression. CON = modified Kreb’s-Henseleit buffer; 5HT = 5 × 10 −7 M 5-HT; ERV = 2.01 × 10 −7 M ERV; 5HT + ERV = 5 × 10 −7 M 5-HT and 2.01 × 10 −7 M ERV. (D) Least squares mean relative HTR2A protein expression ± SEM bovine lateral saphenous veins exposed to control, 5-HT, ERV, or 5-HT + ERV. Probability values for effect of 5-HT: P = 0.68, effect of ERV: P = 0.88, and the interaction of 5-HT and ERV: P = 0.54. ( n = 4).
    Mouse Htr2a Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+htr2a+monoclonal+antibody/SR-2A+Antibody/pmc12741281-105-10-15
    Average 95 stars, based on 59 article reviews
    mouse htr2a monoclonal antibody - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "Association of serotonin and ergot alkaloids on tissue partitioning and contractile response of bovine blood vessels ☆ "

    Article Title: Association of serotonin and ergot alkaloids on tissue partitioning and contractile response of bovine blood vessels ☆

    Journal: Current Research in Toxicology

    doi: 10.1016/j.crtox.2025.100272

    (A) Representative image of HTR2A protein immunoblot with increasing amounts of total protein (10 μg, 12 μg, 14 μg, 16 μg, 18 μg, 20 μg). (B) Linear regression of pooled total protein extracted from bovine lateral saphenous veins with HTR2A protein expression. (B) Representative image of HTR2A protein immunoblot with increasing amounts of total protein. (C) Representative image of HTR2A protein immunoblot for quantitative protein expression. CON = modified Kreb’s-Henseleit buffer; 5HT = 5 × 10 −7 M 5-HT; ERV = 2.01 × 10 −7 M ERV; 5HT + ERV = 5 × 10 −7 M 5-HT and 2.01 × 10 −7 M ERV. (D) Least squares mean relative HTR2A protein expression ± SEM bovine lateral saphenous veins exposed to control, 5-HT, ERV, or 5-HT + ERV. Probability values for effect of 5-HT: P = 0.68, effect of ERV: P = 0.88, and the interaction of 5-HT and ERV: P = 0.54. ( n = 4).
    Figure Legend Snippet: (A) Representative image of HTR2A protein immunoblot with increasing amounts of total protein (10 μg, 12 μg, 14 μg, 16 μg, 18 μg, 20 μg). (B) Linear regression of pooled total protein extracted from bovine lateral saphenous veins with HTR2A protein expression. (B) Representative image of HTR2A protein immunoblot with increasing amounts of total protein. (C) Representative image of HTR2A protein immunoblot for quantitative protein expression. CON = modified Kreb’s-Henseleit buffer; 5HT = 5 × 10 −7 M 5-HT; ERV = 2.01 × 10 −7 M ERV; 5HT + ERV = 5 × 10 −7 M 5-HT and 2.01 × 10 −7 M ERV. (D) Least squares mean relative HTR2A protein expression ± SEM bovine lateral saphenous veins exposed to control, 5-HT, ERV, or 5-HT + ERV. Probability values for effect of 5-HT: P = 0.68, effect of ERV: P = 0.88, and the interaction of 5-HT and ERV: P = 0.54. ( n = 4).

    Techniques Used: Western Blot, Expressing, Modification, Control

    Related Articles

    Incubation:

    Article Title: Association of serotonin and ergot alkaloids on tissue partitioning and contractile response of bovine blood vessels
    Article Snippet: Membranes were blocked for 1 h with 5 % non-fat dry milk with orbital shaking (The Belly Button; Sigma-Aldrich) and then washed three times with Tris-buffered saline with Tween 20 (TBST) in 5-min intervals. .. Membranes were incubated for overnight at 4 °C with 1:333 mouse HTR2A monoclonal antibody (sc-166775; Santa Cruz Biotechnology Inc.). .. After primary antibody incubation, membranes were washed four times with TBST in 5-min intervals and incubated with 1:5000 goat anti-mouse Alexa Fluor Plus 647 secondary antibody (A32728; ThermoFisher Scientific Inc.) for 1 h. After secondary antibody incubation, membranes were washed three times with TBST in 5-min intervals and blots were imaged (Azure Biosystems c600 System) using RGB-green and RGB-red fluorescence (526 nm and 647 nm).

    Article Title: Association of serotonin and ergot alkaloids on tissue partitioning and contractile response of bovine blood vessels.
    Article Snippet: Membranes were blocked for 1 h with 5 % non-fat dry milk with orbital shaking (The Belly Button; Sigma-Aldrich) and then washed three times with Tris-buffered saline with Tween 20 (TBST) in 5-min intervals. .. Membranes were incubated for overnight at 4 ◦C with 1:333 mouse HTR2A monoclonal antibody (sc-166775; Santa Cruz Biotechnology Inc.). .. After primary antibody incubation, membranes were washed four times with TBST in 5-min intervals and incubated with 1:5000 goat anti-mouse Alexa Fluor Plus 647 secondary antibody (A32728; ThermoFisher Scientific Inc.) for 1 h. After secondary antibody incubation, membranes were washed three times with TBST in 5-min intervals and blots were imaged (Azure Biosystems c600 System) using RGB-green and RGB-red fluorescence (526 nm and 647 nm).



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    mouse htr2a monoclonal antibody  (Santa Cruz Biotechnology)
    95
    Santa Cruz Biotechnology mouse htr2a monoclonal antibody
    (A) Representative image of <t>HTR2A</t> protein immunoblot with increasing amounts of total protein (10 μg, 12 μg, 14 μg, 16 μg, 18 μg, 20 μg). (B) Linear regression of pooled total protein extracted from bovine lateral saphenous veins with HTR2A protein expression. (B) Representative image of HTR2A protein immunoblot with increasing amounts of total protein. (C) Representative image of HTR2A protein immunoblot for quantitative protein expression. CON = modified Kreb’s-Henseleit buffer; 5HT = 5 × 10 −7 M 5-HT; ERV = 2.01 × 10 −7 M ERV; 5HT + ERV = 5 × 10 −7 M 5-HT and 2.01 × 10 −7 M ERV. (D) Least squares mean relative HTR2A protein expression ± SEM bovine lateral saphenous veins exposed to control, 5-HT, ERV, or 5-HT + ERV. Probability values for effect of 5-HT: P = 0.68, effect of ERV: P = 0.88, and the interaction of 5-HT and ERV: P = 0.54. ( n = 4).
    Mouse Htr2a Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+htr2a+monoclonal+antibody/SR-2A+Antibody/pmc12741281-105-10-15
    Average 95 stars, based on 1 article reviews
    mouse htr2a monoclonal antibody - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Representative image of HTR2A protein immunoblot with increasing amounts of total protein (10 μg, 12 μg, 14 μg, 16 μg, 18 μg, 20 μg). (B) Linear regression of pooled total protein extracted from bovine lateral saphenous veins with HTR2A protein expression. (B) Representative image of HTR2A protein immunoblot with increasing amounts of total protein. (C) Representative image of HTR2A protein immunoblot for quantitative protein expression. CON = modified Kreb’s-Henseleit buffer; 5HT = 5 × 10 −7 M 5-HT; ERV = 2.01 × 10 −7 M ERV; 5HT + ERV = 5 × 10 −7 M 5-HT and 2.01 × 10 −7 M ERV. (D) Least squares mean relative HTR2A protein expression ± SEM bovine lateral saphenous veins exposed to control, 5-HT, ERV, or 5-HT + ERV. Probability values for effect of 5-HT: P = 0.68, effect of ERV: P = 0.88, and the interaction of 5-HT and ERV: P = 0.54. ( n = 4).

    Journal: Current Research in Toxicology

    Article Title: Association of serotonin and ergot alkaloids on tissue partitioning and contractile response of bovine blood vessels ☆

    doi: 10.1016/j.crtox.2025.100272

    Figure Lengend Snippet: (A) Representative image of HTR2A protein immunoblot with increasing amounts of total protein (10 μg, 12 μg, 14 μg, 16 μg, 18 μg, 20 μg). (B) Linear regression of pooled total protein extracted from bovine lateral saphenous veins with HTR2A protein expression. (B) Representative image of HTR2A protein immunoblot with increasing amounts of total protein. (C) Representative image of HTR2A protein immunoblot for quantitative protein expression. CON = modified Kreb’s-Henseleit buffer; 5HT = 5 × 10 −7 M 5-HT; ERV = 2.01 × 10 −7 M ERV; 5HT + ERV = 5 × 10 −7 M 5-HT and 2.01 × 10 −7 M ERV. (D) Least squares mean relative HTR2A protein expression ± SEM bovine lateral saphenous veins exposed to control, 5-HT, ERV, or 5-HT + ERV. Probability values for effect of 5-HT: P = 0.68, effect of ERV: P = 0.88, and the interaction of 5-HT and ERV: P = 0.54. ( n = 4).

    Article Snippet: Membranes were incubated for overnight at 4 °C with 1:333 mouse HTR2A monoclonal antibody (sc-166775; Santa Cruz Biotechnology Inc.).

    Techniques: Western Blot, Expressing, Modification, Control